文献：磷酸化eIF4E结合蛋白抗体介绍

中文名称    磷酸化eIF4E结合蛋白抗体    

英文名称    phospho-eIF4EBP1 (Ser65 + Thr70)    

别    名    eIF4EBP1 (phospho S65/Th70); eIF4EBP1 (phospho Ser65/Thr70); p-eIF4EBP1 (Ser65/Thr70); eIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E BP1; 4EBP1; BP 1; BP1; eIF4E binding protein 1; eIF4E-binding protein 1; Eukaryotic translation initiation factor 4E binding protein 1; MGC4316; PHAS I; PHASI; PHAS-I; PHAS; 4E-BP1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; Phosphorylated heat and acid stable protein regulated by insulin 1; 4EBP1_HUMAN.    

供 应 商    远慕生物

产品类型    磷酸化抗体     

研究领域    肿瘤  细胞生物  免疫学  信号转导  细胞凋亡  转录调节因子  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 磷酸化eIF4E结合蛋白抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    13kDa    

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from mouse 4EBP1 around the phosphorylation site of Ser65/Thr70    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化eIF4E结合蛋白抗体产品介绍    background:

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008].

Function:
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Subunit:
Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts with RPTOR.

Post-translational modifications:
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1.

Similarity:
Belongs to the eIF4E-binding protein family.

Gene ID:
1978

Database links:

Entrez Gene: 1978 Human

Entrez Gene: 13685 Mouse

Entrez Gene: 116636 Rat

Omim: 602223 Human

SwissProt: Q13541 Human

SwissProt: Q60876 Mouse

SwissProt: Q62622 Rat

Unigene: 411641 Human

Unigene: 6700 Mouse

Unigene: 11161 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.