刺痛感蛋白抗体应用方法

英文名称    Prickle   

中文名称    刺痛感蛋白抗体   

别    名    FLJ31627; FLJ31937; MGC138902; MGC138903; EPM1B; Prickle 1; Prickle homolog 1 (Drosophila); Prickle like protein 1; Prickle-like 1; Prickle1; REST-Interacting LIM Domain Protein; REST/NRSF interacting LIM domain protein 1; RILP; PRIC1_HUMAN.   

供 应 商   远慕生物 

研究领域    转录调节因子  锌指蛋白  表观遗传学     

抗体来源    Rabbit   

克隆类型    Polyclonal   

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,    

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 刺痛感蛋白抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.   

分 子 量    94kDa   

细胞定位    细胞核 细胞膜    

性    状    Lyophilized or Liquid   

浓    度    1mg/1ml   

免 疫 原    KLH conjugated synthetic peptide derived from human Prickle   

亚    型    IgG   

纯化方法    affinity purified by Protein A   

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4   

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.   

刺痛感蛋白抗体产品介绍    background:

Prickle1 is an 831 amino acid protein that contains one PET domain and three LIM zinc-binding domains and localizes to the cytoplasm, as well as to the nuclear membrane. Expressed at higher levels in placenta and at lower levels in liver, brain, kidney, lung and pancreas, Prickle1 is thought to function as a nuclear receptor that interacts with NRSF, a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element). Defects in the gene encoding Prickle1 are associated with autosomal recessive progressive myoclonic epilepsy-1B, which is characterized by quick jerks of the arms, shoulders or legs. The gene encoding Prickle1 maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome.

Function:
Prickle is a LIM domain protein that appears to serve as a nuclear receptor for REST/NRSF, REST4, and possibly other transcription factors. The human protein is a 831 amino acid protein containing 3 N-terminal LIM domains and 3 C-terminal nuclear localization signals. It also contains 4 N-glycosylation sites, 2 PKA phosphorylation sites, and a C-terminal CIIS prenylation motif.

Subunit:
Interacts with REST.

Subcellular Location:
Nuclear Membrane

Tissue Specificity:
Expressed at highest levels in placenta and at lower levels in lung, liver, kidney and pancreas. Expressed in thalamus, hippocampus, cerebral cortex, and cerebellum (in neurons rather than glia).

DISEASE:
Defects in PRICKLE1 are the cause of progressive myoclonic epilepsy type 1B (EPM1B) [MIM:612437]. EPM1B is an autosomal recessive disorder characterized by myoclonus that progresses in severity over time, tonic-clonic seizures and ataxia.
Defects in PRICKLE1 may be a cause of susceptibility to neural tube defects (NTD) [MIM:182940]. Congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy. Failure of neural tube closure can occur at any level of the embryonic axis. Common NTD forms include anencephaly, myelomeningocele and spina bifida, which result from the failure of fusion in the cranial and spinal region of the neural tube. NTDs have a multifactorial etiology encompassing both genetic and environmental components.

Similarity:
Belongs to the prickle / espinas / testin family.
Contains 3 LIM zinc-binding domains.
Contains 1 PET domain.