丝裂原活化蛋白激酶磷酸酶-1抗体说明书

英文名称    MKP1   

中文名称    丝裂原活化蛋白激酶磷酸酶-1抗体

别    名    DUSP1; Mitogen activated protein kinase phosphatase 1; CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUSP 1; DUSP-1; EC 3.1.3.16; EC 3.1.3.48; HVH 1; HVH1; MAP kinase phosphatase 1; MKP 1; MKP1; MKP-1; Protein tyrosine phosphatase CL100; PTPN 10; PTPN10; Serine/threonine specific protein phosphatase; VH 1; VH1; 3ch134; Mkp1; Ptpn16.

供 应 商    远慕生物

研究领域    肿瘤  免疫学  信号转导  细胞凋亡  转录调节因子

抗体来源    Rabbit

克隆类型    Polyclonal

交叉反应    Human, Mouse, Rat, Cow, Horse, Sheep,

产品应用    WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量    39kDa

细胞定位    细胞核

性    状    Lyophilized or Liquid

浓    度    1mg/ml

免 疫 原    KLH conjugated synthetic peptide derived from human MKP1:101-200/367

亚    型    IgG

纯化方法    affinity purified by Protein A

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

丝裂原活化蛋白激酶磷酸酶-1抗体产品说明

MKPs是一类丝/氨酸/苏氨/酸和酪氨/酸双重底物特异性的磷酸酶 ,对于丝裂素活化蛋白激酶活性的调节起着十分重要的作用，可使丝裂素活化蛋白激酶上的苏氨/酸/酪氨/酸去磷酸化失活。目前研究发现MKPs分别有MKP-1、MKP-2、MKP-3及MKP4-6。

MKPs受MAPK信号通路中多种成分的诱导 ,决定了它与MAPK之间作用的特异性。 通过去磷酸化作用调节MAPK信号途径的活性 ,确保了细胞内信号的精确传递 ,参与了多种主要的细胞功能的调节。

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.

mol wt:40kDa

Other Aliases:

Mitogen activated protein kinase phosphatase 1; CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUSP 1; EC 3.1.3.16; EC 3.1.3.48; HVH 1; HVH1; MAP kinase phosphatase 1; MKP 1; MKP1; Protein tyrosine phosphatase CL100; PTPN 10; PTPN10; Serine/threonine specific protein phosphatase; VH 1; VH1; 3ch134; Mkp1; Ptpn16