单核细胞趋化蛋白5抗体说明书

产品名称：单核细胞趋化蛋白5抗体

英文名称：Anti-CCL12/MCP5

别名：C-C motif chemokine 12; CCL12; MCP 5; MCP-5; Macrophage/monocyte Chemotactic Protein; OTT

交叉反应：hu, mo, rat, Rb, dog, hrs, cow

产品类型：一抗

应用领域:/肿瘤/免疫学/转录调节因子

蛋白分子：predicted molecular weight: 11kDa

浓度值：1mg/1ml

物理性状：Lyophilized or Liquid

免疫原：KLH conjugated synthetic peptide derived from human MCP-5 C-terminus

亚型：polyclonal

纯化方法：affinity purified by Protein A

保存液：0.01M PBS, pH 7.4 with 10 mg/ml BSA ａnd 0.1% Sodium azide

应用技术：//WB=1:100-500//ELISA=1:500-1000//IP=1:20-100//IHC-P=1:100-500//IHC-F=1:100-500//IF=1:100-500（石蜡切片需做抗原修复）/not yet tested in other applications./optimal dilutions/concentrations should be determined by the end user.

保存环境：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品介绍：Chemotactic factor that attracts monocytes, lymphocytes, basophils ａnd eosinophils, but not neutrophils. Signals through CCR2B ａnd CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic ａnd non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

抗体,也叫免疫球蛋白 (Ig),是一种能特异性结合抗原的糖蛋白,而抗原是在易感染动物体内引发抗体产生的物质。在体内,抗体是由于外 源性分子的侵袭而产生的。抗体以一个或者多个 Y 字形单体存在,每个 Y 字形单体由 4 条多肽链组成,包含两条相同的重链和两条相同的轻 链。轻链和重链是根据它们的分子量大小来命名的。Y 字形结构的顶端是可变区,为抗原结合部位。

任何一个抗体的轻链都可以分为昊螂型(基于小分子多肽结构上的差异),每一个抗体的重链则决定了它的类或型。抗体制备是指通向抗体生成的步骤，包括抗原样品的制备以及将其安全注入实验动物或家畜体内，并由此诱发抗原特异性抗体在血清内达到高表达水平，然后可从该动物体内回收抗体。